Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Subcutaneous Injections without Adjuvant into Mice and Rats

(Protocol summary only for purposes of this preview site)

Subcutaneous injections can be used to deliver live cells. These immunizations are used to introduce live mammalian cells into the animal in order to prepare anti-cell-surface antibodies. If it can be avoided, do not inject live viruses, bacteria, or fungi because the entire mouse colony might become infected. These organisms are commonly killed or inactivated before injection. Typically, it is not necessary to use adjuvant with whole-cell injections.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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