Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



Table of Contents

Expand All | Contract All

Using Phage Display to Create Recombinant Antibodies

(Protocol summary only for purposes of this preview site)

A variety of phage display technologies have been developed since the approach was first described for antibodies (Huse et al. 1989). Huse et al. followed the pioneering work of George P. Smith (Smith 1985), who described the presentation of antigens on filamentous phage. The most widely used approaches incorporate antibody sequences into the minor coat protein pIII of the nonlytic filamentous phage fd or M13. This was based initially on the work of McCafferty et al. (1990). Libraries of variable gene sequences, encoding either scFv or Fab fragments, are made by incorporation of sequences into phagemid vectors. The phagemid is packaged into phage particles with the assistance of a helper phage to produce the antibody display phage.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

Search for information about other protocols included in the book: